Methylation of high molecular weight fibroblast growth factor-2 determines post-translational increases in molecular weight and affects its intracellular distribution.

Human brain tumor-associated urinary high molecular weight transforming growth factor: a high molecular weight form of epidermal growth factor.

Urinary protein obtained from a patient with a highly malignant brain tumor (astrocytoma, grade IV) was adsorbed to trimethylsilyl controlled-pore glass beads and selectively eluted with acetonitrile to yield a high molecular weight (HMW) human transforming growth factor (hTGF). This HMW hTGF promoted clonogenic cell growth in soft agar and competed for membrane receptors with mouse epidermal g...

Differential modulation of cell phenotype by different molecular weight forms of basic fibroblast growth factor: possible intracellular signaling by the high molecular weight forms

To study possible functional differences of the 18-kD and high molecular weight forms of basic fibroblast growth factor (bFGF), we have examined the effect of endogenous production of different bFGF forms on the phenotype of NIH 3T3 cells. Cells transfected with cDNAs coding for either 18-kD bFGF (18-kD bFGF) or all four molecular forms (18, 22, 22.5, 24 kD; wild type [WT] bFGF) exhibit increas...

Epidermal growth factor: high and low molecular weight forms.

In crude homogenates of the submaxillary gland of the male mouse, epidermal growth factor (EGF) activity was found almost entirely in a high molecular weight complex (about 74,000 daltons). This high molecular weight form may be reversibly dissociated into EGF and an EGF-binding protein. EGF was found to have a molecular weight of approximately 6400. The EGF-binding protein has a molecular weig...

Synthesis of high molecular weight polyethylene using FI catalyst

A FI Zr-based catalyst of bis[N-(3,5-dicumylsalicylidene)-2′,6′­diisopropylanilinato]zirconium(IV) dichloride was prepared and used for polymerization of ethylene. The effects of reaction conditions on the polymerization were examined in detail. The increase in ethylene pressure and rise in polymerization temperature up to 35 oC were favorable for catalyst/MAO to raise the catalytic activity as...

Rheological Method for Determining Molecular Weight and Molecular Weight Distribution